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erβ antagonist  (Tocris)
94
Tocris erβ antagonist
Fig. 4. 27HC activates POMC neurons <t>via</t> <t>ERα.</t> (A to L) Immunofluorescent staining for c-Fos (red) in the arcuate nucleus of the hypothalamus (ARH) of female [(A) to (F)] and male [(G) to (L)] ERα-ZsGreen mice 90 min after a single bolus ICV infusion of vehicle or 27HC (0.1 nmol). White arrows point to dual-colored neurons with ERα (green) and c-Fos (red) expressed. (M and N) Summary of quantification per section of female and male mice (n = 3). (O) Schematic of POMC neurons in the ARH (POMCARH) treated with 27HC for electrophysiology recordings. (P) A micrographic image showing a recorded POMCARH (red) neuron of male mice POMC-Cre/Rosa26-LSL-tdTOMATO mice. (Q) Resting membrane potential (RM) of POMCARH neurons before and after 27HC treatment. (R) Summary of the responsive ratio of POMCARH neurons for all doses of 27HC. (S and T) The effects of 100 nM 27HC on RM before and after ERα antagonist BHPI (S) or <t>ERβ</t> antagonist PHTPP (T) incubation. The brain slices were first preincubated with presynaptic inhibitors 1 μM TTX (a voltage-gated sodium channel blocker) + 30 μM CNQX (an AMPA receptor antagonist) + 30 μM DAP-5 (an NMDA receptor antagonist) + 50 μM bicuculline (a GABA receptor antagonist). Results are shown as means ± SEM. [(M) and (N)] *P < 0.05 indicates statistical significance in unpaired t tests. [(Q), (S), and (T)] **P < 0.01 and ****P < 0.0001 indicate statistical significance in paired t tests.
Erβ Antagonist, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phtpp+tocris/PHTPP/pm38996023-430-52-57
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erβ antagonist - by Bioz Stars, 2026-09
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phtpp  (Bio-Techne corporation)
95
Bio-Techne corporation phtpp
Fig. 4. 27HC activates POMC neurons <t>via</t> <t>ERα.</t> (A to L) Immunofluorescent staining for c-Fos (red) in the arcuate nucleus of the hypothalamus (ARH) of female [(A) to (F)] and male [(G) to (L)] ERα-ZsGreen mice 90 min after a single bolus ICV infusion of vehicle or 27HC (0.1 nmol). White arrows point to dual-colored neurons with ERα (green) and c-Fos (red) expressed. (M and N) Summary of quantification per section of female and male mice (n = 3). (O) Schematic of POMC neurons in the ARH (POMCARH) treated with 27HC for electrophysiology recordings. (P) A micrographic image showing a recorded POMCARH (red) neuron of male mice POMC-Cre/Rosa26-LSL-tdTOMATO mice. (Q) Resting membrane potential (RM) of POMCARH neurons before and after 27HC treatment. (R) Summary of the responsive ratio of POMCARH neurons for all doses of 27HC. (S and T) The effects of 100 nM 27HC on RM before and after ERα antagonist BHPI (S) or <t>ERβ</t> antagonist PHTPP (T) incubation. The brain slices were first preincubated with presynaptic inhibitors 1 μM TTX (a voltage-gated sodium channel blocker) + 30 μM CNQX (an AMPA receptor antagonist) + 30 μM DAP-5 (an NMDA receptor antagonist) + 50 μM bicuculline (a GABA receptor antagonist). Results are shown as means ± SEM. [(M) and (N)] *P < 0.05 indicates statistical significance in unpaired t tests. [(Q), (S), and (T)] **P < 0.01 and ****P < 0.0001 indicate statistical significance in paired t tests.
Phtpp, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phtpp+tocris/PHTPP/bio-techne+corporation___2662
Average 95 stars, based on 1 article reviews
phtpp - by Bioz Stars, 2026-09
95/100 stars
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Image Search Results


Fig. 4. 27HC activates POMC neurons via ERα. (A to L) Immunofluorescent staining for c-Fos (red) in the arcuate nucleus of the hypothalamus (ARH) of female [(A) to (F)] and male [(G) to (L)] ERα-ZsGreen mice 90 min after a single bolus ICV infusion of vehicle or 27HC (0.1 nmol). White arrows point to dual-colored neurons with ERα (green) and c-Fos (red) expressed. (M and N) Summary of quantification per section of female and male mice (n = 3). (O) Schematic of POMC neurons in the ARH (POMCARH) treated with 27HC for electrophysiology recordings. (P) A micrographic image showing a recorded POMCARH (red) neuron of male mice POMC-Cre/Rosa26-LSL-tdTOMATO mice. (Q) Resting membrane potential (RM) of POMCARH neurons before and after 27HC treatment. (R) Summary of the responsive ratio of POMCARH neurons for all doses of 27HC. (S and T) The effects of 100 nM 27HC on RM before and after ERα antagonist BHPI (S) or ERβ antagonist PHTPP (T) incubation. The brain slices were first preincubated with presynaptic inhibitors 1 μM TTX (a voltage-gated sodium channel blocker) + 30 μM CNQX (an AMPA receptor antagonist) + 30 μM DAP-5 (an NMDA receptor antagonist) + 50 μM bicuculline (a GABA receptor antagonist). Results are shown as means ± SEM. [(M) and (N)] *P < 0.05 indicates statistical significance in unpaired t tests. [(Q), (S), and (T)] **P < 0.01 and ****P < 0.0001 indicate statistical significance in paired t tests.

Journal: Science advances

Article Title: 27-Hydroxycholesterol acts on estrogen receptor α expressed by POMC neurons in the arcuate nucleus to modulate feeding behavior.

doi: 10.1126/sciadv.adi4746

Figure Lengend Snippet: Fig. 4. 27HC activates POMC neurons via ERα. (A to L) Immunofluorescent staining for c-Fos (red) in the arcuate nucleus of the hypothalamus (ARH) of female [(A) to (F)] and male [(G) to (L)] ERα-ZsGreen mice 90 min after a single bolus ICV infusion of vehicle or 27HC (0.1 nmol). White arrows point to dual-colored neurons with ERα (green) and c-Fos (red) expressed. (M and N) Summary of quantification per section of female and male mice (n = 3). (O) Schematic of POMC neurons in the ARH (POMCARH) treated with 27HC for electrophysiology recordings. (P) A micrographic image showing a recorded POMCARH (red) neuron of male mice POMC-Cre/Rosa26-LSL-tdTOMATO mice. (Q) Resting membrane potential (RM) of POMCARH neurons before and after 27HC treatment. (R) Summary of the responsive ratio of POMCARH neurons for all doses of 27HC. (S and T) The effects of 100 nM 27HC on RM before and after ERα antagonist BHPI (S) or ERβ antagonist PHTPP (T) incubation. The brain slices were first preincubated with presynaptic inhibitors 1 μM TTX (a voltage-gated sodium channel blocker) + 30 μM CNQX (an AMPA receptor antagonist) + 30 μM DAP-5 (an NMDA receptor antagonist) + 50 μM bicuculline (a GABA receptor antagonist). Results are shown as means ± SEM. [(M) and (N)] *P < 0.05 indicates statistical significance in unpaired t tests. [(Q), (S), and (T)] **P < 0.01 and ****P < 0.0001 indicate statistical significance in paired t tests.

Article Snippet: To test whether ERα or ERβ is necessary for the stimulatory effects of 27HC on POMCARH neurons, brain slices were treated with 100 nM 27HC in the presence of TTX, a cocktail of synaptic inhibitors, and either BHPI (50 μM), an ERα antagonist (catalog no. 5.38005, MilliporeSigma), or PHTPP (10 μM), an ERβ antagonist (catalog no. 2662, Tocris).

Techniques: Staining, Membrane, Incubation